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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

13 discrete DNA fragments from 100 bp to 3000 bp

The 100 bp DNA Ladder is a ready-to-load molecular weight marker suitable for DNA fragment size determination on gel electrophoresis.

  • thirteen crisp bands
  • stable at room temperature
  • visualization dye included

Ordering

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Catalog number

07-11-00050

Quantity

€ 25.00

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Details

Overview

Solis BioDyne 100 bp DNA Ladder Ready to Load is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 3,000 bp.

100 bp DNA Ladder Ready to Load is composed of thirteen DNA fragments (in base pairs): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 3000. It contains one reference band (500 bp) for easy orientation.

100 bp DNA Ladder Ready to Load contains bromophenol blue dye which serves as visual aid to monitor the progress of migration during agarose gel electrophoresis.

Properties

Concentration: 0.1 µg/µl

Size range: 100 bp - 3 000 bp

No of bands: 13

Storage Solution: 10 mM EDTA, 10% glycerol, 0.015% bromophenol blue and 0.17% SDS.

Related Resources

FAQ

How are DNA samples prepared for gel electrophoresis?

Dilute your DNA sample with the 6x DNA Loading Dye Buffer: mix 1 volume of the Loading Dye Buffer with 5 volumes of your DNA sample.

Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.

For the best results, use Solis BioDyne 6x Loading Dye Buffers (Blue Dye Cat. No. 07-01-00010, Double Blue Dye 07-02-00010, Orange Dye 07-04-00010, Orange Blue Dye 07-03-00010).

Should I add loading dye to the Solis BioDyne DNA Ladders before loading on the gel?

No. Solis Biodyne 100 bp DNA Ladder and 1 kb DNA Ladder are ready-to-use.

Solis Biodyne 100 bp DNA Ladder and 1 kb DNA Ladder are premixed with 6x DNA Loading Dye Buffer Blue (Cat. No. 07-01-00010) and 6x DNA Loading Dye Buffer Double Blue (Cat. No. 07-02-00010), respectively.

What is a DNA ladder in gel electrophoresis?
DNA Ladder is a molecular-weight size marker used to identify the approximate size (usually in base pairs) and amount (usually in nanograms) of a DNA molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel. DNA ladder contains double-stranded DNA fragments whose molecule weights (and size in base pairs) are known and standardized. When run alongside your PCR product in a gel, the DNA ladder allows you to estimate the size and amount of your DNA fragment by comparing it to the closest band in the ladder lane.
How much ladder should I load on the agarose gel?

For a standard electrophoresis system, load 0.4-0.8 µl (0.04-0.08 µg) of Solis BioDyne 100 bp or 1 kb DNA Ladder Ready to Load per 1 mm of gel lane (per 1 mm of well width). It is generally recommended to load the same volumes of your DNA sample and the DNA Ladder.

What is the function of DNA ladder in gel electrophoresis?

A molecular-weight size marker used to identify the approximate size (usually in base pairs) and amount (usually in nanograms) of a DNA molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel.

DNA ladder contains double-stranded DNA fragments whose molecule weights (and size in base pairs) are known and standardized. When run alongside your PCR product in a gel, the DNA ladder allows you to estimate the size and amount of your DNA fragment by comparing it to the closest band in the ladder lane.

Storage & Shipping
Storage

Storage for up to 9 months at room temperature has no detrimental effects on the quality of this reagent.

Long term storage: -20ºC is recommended

Shipping

At room temperature