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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

PCR Master Mix for routine applications

  • suitable for templates up to 5 kb
  • reaction set-up and shipment without dry ice
  • all-in-one master mix format reduces pipetting errors and saves time
  • amplicons are suitable for Sanger sequencing post-PCR

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MgCl2 (mM) final concentration
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Catalog number

04-11-00S15

Quantity

€ 0.00

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Details

Overview

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs and buffer with detergent. You just need to add template, primers and water.

We recommend using 5x FIREPol® Master Mix without ready to load feature in all PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: no

Ready to load: no

Fidelity: same as Taq

Cloning Type: T/A Cloning

Amplicon Size: up to 5 kb

Mix Components

FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

7.5 mM MgCl21x PCR solution – 1.5 mM MgCl2)

1 mM dNTPs of each:  1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Plant genomic DNA
Related Resources

FAQ

What is the function of MgCl₂ in a PCR reaction?

The concentration of the MgCl2 in PCR reaction affects the productivity of your PCR reaction. Higher concentrations of MgCl2 may increase productivity of DNA polymerase, but also may decrease specificity causing unexpected products.

Usually, 1.5-2.5 mM final concentration of MgCl2 is optimal for general PCR. However, optimal concentration of MgCl2 depends on template, buffer, DNA and dNTPs. Solis BioDyne produces Master Mixes at different MgCl2 concentration, which allows greater flexibility in reaction optimisation. For your very first experiment with Master Mix, we recommend to use Master Mix with 1,5 - 2 mM MgCl2 (in final 1x PCR Mix)

What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Publications

First detection of zoonotic tapeworm Echinococcus granulosus sensu lato genotype G7 in continental Italy

T. Laurimäe et al. / Parasitol. Res. / 2019

Towards PacBio-based pan-eukaryote metabarcoding using full-length ITS sequences

L. Tedersoo and S. Anslan / Environ. Microbiol. Rep. / 2019

Boosting the monitoring of phytoplankton in optically complex coastal waters by combining pigment-based chemotaxonomy and in situ radiometry

M. Tamm et al. / Ecol. Indic. / 2019
read more
Storage & Shipping
Storage

Routine storage: -20ºC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® Master Mix

Shipping

At room temperature