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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

For loading DNA samples like PCR fragments and restriction fragments on gel

For loading DNA samples like PCR fragments and restriction fragments on gel. 

  • includes two tracking dyes
  • easy loading and tracking of DNA samples
  • store at room temperature

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Catalog number

07-02-0000S

Quantity

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Details

Overview

Description

6x DNA Loading Dye Buffer Double Blue can be used for loading DNA samples (PCR products, restriction fragments) on agarose or polyacrylamide gel.

The loading dye serves as visual marker of electrophoresis: In 1% agarose gel Bromophenol blue comigrates with ca 300 bp (1 x TBE) or ca 400 bp (1x TAE) fragments and Xylene Cyanole FF comigrates with ca 3500 bp (1x TBE) or ca 5000 bp (1x TAE) fragments.

Properties

Reagent Composition: Bromphenol blue, Xylene Cyanole FF, TRIS-HCl, EDTA and Ficoll

Features: No interaction with the borate on TBE gels is observed, altering local pH due to the Ficoll component

Recommendations: Mix 1 volume ofdouble blue Dye with 5 volumes of your DNA sample and load into the gel

Related Resources

FAQ

What is 6X loading dye buffer?

The 6X Loading Dye buffer is used to prepare DNA ladders and samples for loading on agarose or polyacrylamide gels.

Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.

What percentage agarose is required for electrophoresis?

The concentration of agarose in a gel depends on the sizes of the DNA fragments, and should be able to separate your fragment(s) enough so that they are identifiable.

Smaller fragments of DNA migrate through the gel more quickly and, therefore, are separated on higher concentrations of agarose. Larger molecules require a lower concentration of agarose.

How are DNA samples prepared for gel electrophoresis?

Dilute your DNA sample with the 6x DNA Loading Dye Buffer: mix 1 volume of the Loading Dye Buffer with 5 volumes of your DNA sample.

Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.

For the best results, use Solis BioDyne 6x Loading Dye Buffers (Blue Dye Cat. No. 07-01-00010, Double Blue Dye 07-02-00010, Orange Dye 07-04-00010, Orange Blue Dye 07-03-00010).

Storage & Shipping
Storage

Store at room temperature or at 4ºC.
Long-term storage at -20ºC.

Shipping

At room temperature