Solis BioDyne OÜ A: Teaduspargi 9, 50411 Tartu, Estonia T: +372 740 9960 E: info@solisbiodyne.com SKYPE: solis.biodyne
Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A.
RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor is active over a broad range of reaction temperatures from 37°C to 50°C and has enhanced stability at room temperature with no loss of activity for up to 1 month.
Preventing RNA degradation in the following applications:
Concentration: 40 U/µl
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 10 mM DTT and stabilizers.
Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 1 U/μl. Inactivated by heating at 85°C for 5 min.
Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RigoGrip inhibits Rnase H.
Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
miR-1933-3p is upregulated in skeletal muscles of MuSK+ EAMG mice and affects Impa1 and Mrpl27
Routine storage: -20ºCTemporary storage for up to 1 month at room temperature has no detrimental effects on the quality of RiboGrip™ RNase Inhibitor.
At room temperature
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
Highly sensitive cDNA synthesis and dye-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
FIREScript® KIT contains a powerful genetically modified MMLV-based FIREScript® reverse transcriptase with increased thermostability and exceptional stability at room temperature. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
All-inclusive and the most convenient product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip™) and a reaction premix in separate tubes allowing you to choose the most appropriate priming option.
SOLIScript® KIT contains a unique in silico-designed SOLIScript® reverse transcriptase with high thermostability and exceptional stability at room temperature. SOLIScript® suits best with oligo dT and gene-specific primers. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
A flexible and highly specific cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization. Note! The kit includes oligo dT primers, SOLIScript® is not recommended with random primers.
All-inclusive and the most convenient product format for first-strand cDNA synthesis using gene-specific primers. A combined enzyme mix (SOLIScript® and RiboGrip™) and a reaction premix in separate tubes. Primers not included.