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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Extremely fast and highly sensitive dye-based qPCR

Extremely fast and highly sensitive dye-based qPCR for most demanding applications.

  • qPCR results 2x faster
  • highly sensitive SolisGreen® dye
  • reaction set-up and shipment without dry ice

Ordering

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Catalog number

28-46-00001

Quantity

€ 105.00

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Details

Overview

Description

SolisFAST® SolisGreen® qPCR Mix is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays.

Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.

Properties

Concentration: 5x

Hot-start: yes, 3 minutes initial incubation at 95°C

Cycling speed: fast and ultra-fast

Detection type: dye-based, includes SolisGreen® intercalating dye

Reference dye: based on ROX

Compatible real-time instruments: The mix is compatible with qPCR cyclers where ROX is used as a passive reference signal for normalization of the data. Check Your cycler!

Mix Components:

SolisFAST® DNA PolymeraseIn silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction

Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR

Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel

SolisGreen® dye

This dye-based qPCR mix contains next generation DNA binding dye  SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major realtime cyclers. SolisGreen® dye shows:

  • high fluorescence level
  • high sensitivity for detecting low template concentrations
  • high stability for room temperature storage
Dye-based qPCR results in 30 minutes
Accurate and sensitive qPCR
Related Resources

FAQ

What is the difference between dye-based and probe-based systems?

Dye-based detection (e.g., EvaGreen®, SolisGreen®, SYBR® Green) is a cost-effective qPCR option, as it requires only addition of PCR primers. However, the intercalating dye will detect any dsDNA (non-specific amplicons, primer dimers) produced in the reaction. Melt curve analysis performed after the qPCR can be used to verify the specificity of amplification and to check for the presence of non-specific amplification products.

The probe-based qPCR system demonstrates higher specificity compared to dye-based qPCR, because probes only detect the gene of interest. Keep in mind that in probe-based assays, primer dimers and non-specific products will not be detected, however, they may compromise the PCR efficiency. Using probe-based qPCR system, it is possible to distinguish between sequences with high similarity (e.g. single-nucleotide variations). Additionally, probe-based qPCR assays allow for multiplex reactions in one tube, while only a single target can be amplified and measured in a dye-based qPCR.

I have previously used SYBR® Green / EvaGreen® based qPCR assays. What are the spectral features of SolisGreen™ dye?

SolisGreen™ nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). SolisGreen™ is characterized by high sensitivity and great PCR efficiency with low template amounts for accurate and reproducible results. 

SolisGreen™ is based on CYGREEN dye. CYGREEN is used under licence from Enzo Life Sciences, Inc. CYGREEN is a U.S. registered trademark of Enzo Life Sciences, Inc. U.S. Patent Nos. 8,153,802 and 7,569,695.

After first-strand cDNA synthesis, how much cDNA template should I use for my PCR?

Recommended final amount of cDNA sample in downstream PCR reaction is up to one tenth of the final reaction volume. Overload of cDNA sample may compromise the downstream PCR, because cDNA sample may contain reaction components that may inhibit your PCR reaction.

What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage at -20 °C until expiry date

The mix can be stored at +4 °C for up to 6 months

Stability at room temperature (15 - 25 °C) for 1 month

Freeze-thaw stability: 30 cycles

Shipping

At room temperature