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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

DNA Polymerase for MALDI-TOF and other applications with unconventional nucleotides

Thermostable DNA polymerase for highly efficient incorporation of unconventional nucleotides.

  • efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides
  • the assay success rate of 99% in MALDI-TOF
  • robust and reliable
  • reaction set-up and shipment without dry ice

Ordering

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Catalog number

01-03-0000S

Quantity

€ 0.00

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Details

Reagents Provided
Item Pcs. Vial size
TERMIPol® DNA Polymerase 1 500 U | 100 µl
10x Reaction Buffer C 1 5 ml
100 mM MgCl2 1 5 ml
Overview

Description

TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms.

The enzyme has 5’→ 3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs, acyNTPs, fluorescent dNTPs and labeled ddNTPs).

Applications

  • MALDI-TOF mass spectrometry
  • Primer extension
  • Mass-array

Properties

Concentration: 5 U/µl

Hot-start: no

Error rate per nucleotide per cycle is app. 8 x 10-5

Estimated half life at 95ºC is 1.5 hours.

Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.

Reagents

TERMIPol® DNA Polymerase

10x Reaction buffer C 500 mM Tris-HCl pH 9.5 at 25ºC

100 mM MgCl2

Related Resources

FAQ

Enzymes are usually very viscous liquids. What would you recommend for the most accurate pipetting?

Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:

  • Hold the pipette vertical, since more liquid gets picked up into the tip when the pipette is held at an angle.
  • Only put the pipette tip in the liquid.
  • Use a very slow aspiration speed, wait for a few seconds until the liquid retightens and then use a very slow dispensing speed.
What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of TERMIPol® DNA Polymerase.

Shipping

At room temerature