- First strand cDNA synthesis
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
- SOLIScript Reverse Transcriptase
- RiboGrip RNase Inhibitor
- 10x RT Reaction Buffer with DTT
500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
- Oligo (dT) Primer
- dNTP Mix
- Water, nuclease free
Shipping and Storage Conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
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