SolisFAST® Master Mix with UNG, Ready to Load

Fast singleplex and multiplex endpoint PCR assays

For in vitro use only

 

Description:

SolisFAST® Master Mix with UNG Ready to Load is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
24-22-000011 ml5xPDFPDF174 EUR Login to buy!
24-22-00001-55 x 1 ml5xPDFPDF820 EUR Login to buy!
24-22-0002020 ml5xPDFPDF2880 EUR Login to buy!

Applications:

- Ultra fast endpoint analysis - results in 20 minutes

- Suitable for singleplex and multiplex reactions

- High-yield PCR

- Template generation for sequencing

 

Benefits:

  • FAST - less time from sample to result
  • ACCURATE - specific and reliable PCR in singleplex and multiplex assays
  • STABLE - 30 day stability at room temperature
  • TRUSTWORTHY - avoid carry-over contamination with UNG mixes and get reliable results in sanger sequencing applications

  

Mix Composition:

- SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

- PCR buffer: Includes 17.5 mM MgCl2 (1X PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR reaction

- Green loading dye*: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.

*The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).

 

Shipping and Storage Conditions:

  • Routine storage at -20 °C until expiry date
  • The mix can be stored at +4 °C for up to 6 months
  • Stability at room temperature (15 - 25 °C) for 1 month
  • Freeze-thaw stability: 30 cycles

 

This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ.
Please contact support@solisbiodyne.com for any questions or comments regarding the performance of the Mix.

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.

Contact:

Solis BioDyne OÜ
Reg no: 10242922

Teaduspargi 9
50411 Tartu
Estonia

E-mail: info@solisbiodyne.com

Tel: +372 740 9960

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