5x HOT FIREPol® EvaGreen® HRM Mix

For in vitro use only



5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.


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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-31-000011 ml5xPDFPDFPDF44 EUR Login to buy!
08-31-000088 ml5xPDFPDFPDF332 EUR Login to buy!
08-31-0002020 ml5xPDFPDFPDF760 EUR Login to buy!


High Resolution Melt (HRM)


Mix Compatibility:

- Bio-Rad: CFX96™ & CFX384™

- Qiagen: Rotor-Gene® 6000

- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4

- Illumina: The Eco™

- Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® HRM buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM 

- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP 

- EvaGreen® dye



EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior for HRM. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!


Shipping and Storage conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® HRM Mix.


EvaGreen® is a registered trademark of BIOTIUM, INC. 
This product is provided under an agreement between BIOTIUM, INC. and SBD and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis.  The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.  Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research.  For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.”

Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384 are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.
Please browse through our Troubleshooting Guide for qPCR

send an e-mail directly to our technical support or 

contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

References of HOT FIREPol® EvaGreen® HRM Mix

Association of common variants in PAH and LAT1 with non-syndromic cleft lip with or without cleft palate (NSCL/P) in the Polish population
Hozyasz KK, Mostowska A, Wójcicki P, Lasota A, Wołkowicz A, Dunin-Wilczyńska I, Jagodziński PP
Archives of Oral Biology 2014, Vol. 59. Iss 4. P. 363-369

Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice
Ramirez MC, Zubeldía-Brenner L, Wargon V, Ornstein AM, Becu-Villalobos D
Molecular and Cellular Endocrinology 2014, Vol 382, Iss 2, P. 825-834

The effect of CD14 and TLR4 gene polimorphisms on asthma phenotypes in adult Turkish asthma patients: a genetic study
Şahin F, Yıldız P, Kuskucu A, Kuskucu MA, Karaca N, Midilli K
BMC Pulmonary Medicine 2014, 14:20

Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer
Rawluszko AA, Bujnicka KE, Horbacka K, Krokowicz P, Jagodziński PP
BMC Cancer 2013, 13:526

An innovative tool for moving malaria PCR detection of parasite reservoir into the field
Canier L et al.
Malaria Journal 2013, 12:405 

Performance of “VIKIA Malaria Ag Pf/Pan” (IMACCESSW), a new malaria rapid diagnostic test
for detection of symptomatic malaria infections
Chou M, Kim S, Khim N, Chy S, Sum S, Dourng D, Canier L, Nguon C, Ménard D
Malaria Journal 2012, 11:295

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.


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