Applications:
High Resolution Melt (HRM)
Mix Compatibility:
- Bio-Rad: CFX96™ & CFX384™
- Qiagen: Rotor-Gene® 6000
- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4
- Illumina: The Eco™
- Roche: LightCycler® 480
Mix Composition:
- HOT FIREPol® DNA Polymerase
- 5x EvaGreen® HRM buffer
- 12.5 mM MgCl2 1x PCR solution – 2.5 mM
- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- EvaGreen® dye
- BSA
EvaGreen® Dye:
EvaGreen® is a DNA-binding dye with many features that make it a superior for HRM. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® HRM Mix.
EvaGreen® is a registered trademark of BIOTIUM, INC.
This product is provided under an agreement between BIOTIUM, INC.
and SBD and the manufacture, use, sale or import of this product is
subject to one or more of pending patent applications owned by BIOTIUM
and ALLELOGIC. The purchase of this product conveys to the buyer the
non-transferable right to use the purchased amount of the product and
components of the product in research conducted by the buyer, where such
research does not include testing, analysis or screening services for
any third party in return for compensation on a per test basis. The
buyer cannot sell or otherwise transfer (a) this product (b) its
components or (c) materials made using this product or its components to
a third party or otherwise use this product or its components or
materials made using this product or its components for Commercial
Purposes. Commercial Purposes means any activity by a party for
consideration and may include, but is not limited to: (1) use of the
product or its components in manufacturing; (2) use of the product or
its components to provide a service, information, or data; (3) use of
the product or its components for therapeutic, diagnostic or
prophylactic purposes; or (4) resale of the product or its components,
whether or not such product or its components are resold for use in
research. For information on purchasing a license to this product for
purposes other than research, contact BIOTIUM, INC., Business
Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel:
510-265-1027, Fax: 510-265-1352.”
Mastercycler is a trademark of
Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research
(Qiagen). CFX96, CFX384 are
trademarks of Bio-Rad Laboratories. LightCycler is a registered
trademark of Roche Applied Sciences. The Eco is registered trademark of
Illumina Inc.
Please browse through our
Troubleshooting Guide for qPCR,
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.
References of HOT FIREPol® EvaGreen® HRM Mix
Association of common variants in PAH and LAT1 with non-syndromic cleft lip with or without cleft palate (NSCL/P) in the Polish population
Hozyasz KK, Mostowska A, Wójcicki P, Lasota A, Wołkowicz A, Dunin-Wilczyńska I, Jagodziński PP
Archives of Oral Biology 2014, Vol. 59. Iss 4. P. 363-369
Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice
Ramirez MC, Zubeldía-Brenner L, Wargon V, Ornstein AM, Becu-Villalobos D
Molecular and Cellular Endocrinology 2014, Vol 382, Iss 2, P. 825-834
The effect of CD14 and TLR4 gene polimorphisms on asthma phenotypes in adult Turkish asthma patients: a genetic study
Şahin F, Yıldız P, Kuskucu A, Kuskucu MA, Karaca N, Midilli K
BMC Pulmonary Medicine 2014, 14:20
Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer
Rawluszko AA, Bujnicka KE, Horbacka K, Krokowicz P, Jagodziński PP
BMC Cancer 2013, 13:526
An innovative tool for moving malaria PCR detection of parasite reservoir into the field
Canier L et al.
Malaria Journal 2013, 12:405
Performance of “VIKIA Malaria Ag Pf/Pan” (IMACCESSW), a new malaria rapid diagnostic test
for detection of symptomatic malaria infections
Chou M, Kim S, Khim N, Chy S, Sum S, Dourng D, Canier L, Nguon C, Ménard D
Malaria Journal 2012, 11:295