Hot Start PCR
Hot Start PCR prevents misextension from non-specifically annealed primers and primer-dimers.
HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. At ambient temperature it is inactive having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 12-15 min incubation step at 95º C
Hot-start master mixes are also available in ready-to-load format with variety of MgCl2 concentrations.
5x HOT FIREPol® Blend Master Mix is a highly
user-friendly and versatile tool for your Hot Start PCR. The mix is
based on a blend of our HOT FIREPol® polymerase and a proofreading
polymerase, providing a low error rate and high sensitivity. In addition,
the mix also allows amplification of longer fragments (up to 5 kb). Here some
great advantages of this ready-to-load mix:
- reduced risk of contamination
- consistent reaction to reaction performance
- contains all reagents required for PCR
Like all our products, the 5x HOT FIREPol® Blend Master Mix is
stable at ambient temperature for up to one month. This makes handling and
set-up even more convenient, since no ice has to be used.