Applications:
- Hot Start PCR
Mix Composition:
- HOT FIREPol® DNA polymerase
- Proofreading enzyme
- 5x Blend Master Mix Buffer
- 10 mM MgCl2 1x PCR solution – 2 mM MgCl2
- 1 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
- BSA
- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment
- Yellow dye Migration rate in excess of primers in 1% agarose gel: <35-45 bp
- Compound that increases sample density for direct loading
Advantages of Ready-to-Load Mix:
- decreased set-up time
- reduced risk of contamination
- less pipetting mistakes
- consistent reaction to reaction performance
- contains all reagents required for PCR
Like all our products, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 12 months at 2-8ºC has no detrimental effects on the quality of 5x HOT FIREPol® Blend Master Mix Ready to Load.
Please browse through our
Troubleshooting Guide for End-Point PCR,
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.
References of HOT FIREPol® Blend Master Mix
Dothistroma septosporum on firs (Abies spp.) in the northern Baltics
Drenkhan R, Adamson K, Jürimaa K, Hanso M
Forest Pathology, June 2014. Vol 44, Iss 3, pp. 250-254
Issues in Identifying Germ Tube Positive Yeasts by Conventional Methods
Yazdanpanah A, Khaithir TMN
J. of Clin. Lab. Anal. 2014, Vol. 28, Iss1, P. 1-9.
Identification and ß-lactam resistance in aquatic isolates of Enterobacter cloacae and their status in microbiota of Domica Cave in Slovak Karst (Slovakia)
Gaalova B, Donauerova A, Seman M, Bujdakova H
International Journal of Speleology, 2014, Vol. 43, Iss. 1
Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant Klebsiella pneumoniae Isolate
Al-Marzooq F, Yusof MYM, Tay ST
Jpn. J. Infect. Dis., 2013, Vol 66, No 6, p 555-557
Development and Characterization of Genomic and Gene-Based Microsatellite Markers in North American Red Oak Species
Sullivan AR, Lind JF, McCleary TS, Romero-Severson J, Gailing O
Plant Mol. Biol. Rep. 2013, Vol. 31, pp 231-239
Phylogenetic relationships among host plants explain differences in fungal species richness and community composition in ectomycorrhizal symbiosis
Tedersoo L, Mett M, Ishida TA, Bahram M
New Phytologist, 2013, DOI: 10: 1111/nph. 12328
Comparison of the levels of Equus caballus papillomavirus type 2 (EcPV-2) DNA in equine squamous cell carcinomas and non-cancerous tissues using quantitative PCR
Knighta CG, Dunowska M, Munday JS, Peters-Kennedy J, Rosa BV
Vetmic. 2013 Vol 166, Iss 1-2, P. 257-262