5x HOT FIREPol® Blend Master Mix Ready to Load

with 7.5 mM MgCl2

For in vitro use only 


5x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase. 

In addition, the mix also allows amplification of longer fragments

Generated PCR products are compatible with blunt-end and TA cloning procedures (to increase the blunt end cloning efficiency treat the PCR products with T4 DNA polymerase or DNA polymerase I large Klenow fragment prior to cloning).

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
04-25-001151 ml5xPDFPDFPDF34 EUR Login to buy!
04-25-0201520 ml5xPDFPDFPDF590 EUR Login to buy!


- Hot Start PCR


Mix Composition:

- HOT FIREPol® DNA polymerase

- Proofreading enzyme

- 5x Blend Master Mix Buffer

- 7. 5 mM MgCl2  1x PCR solution – 1.5 mM MgCl2

- 1 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP


- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

- Yellow dye Migration rate in excess of primers in 1% agarose gel: <35-45 bp

- Compound that increases sample density for direct loading

Advantages of Ready-To-Load Mix:

- decreased set-up time

- reduced risk of contamination

- less pipetting mistakes

- consistent reaction to reaction performance

- contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.


Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 12 months at 2-8ºC has no detrimental effects on the quality of 5x HOT FIREPol® Blend Master Mix Ready to Load.


Please browse through our Troubleshooting Guide for End-Point PCR
send an e-mail directly to our technical support or 
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.


References of HOT FIREPol® Blend Master Mix

Dothistroma septosporum on firs (Abies spp.) in the northern Baltics
Drenkhan R, Adamson K, Jürimaa K,  Hanso M
Forest Pathology, June 2014. Vol 44, Iss 3, pp. 250-254
Issues in Identifying Germ Tube Positive Yeasts by Conventional Methods
Yazdanpanah A, Khaithir TMN
J. of Clin. Lab. Anal. 2014, Vol. 28, Iss1, P. 1-9.
Identification and ß-lactam resistance in aquatic isolates of Enterobacter cloacae and their status in microbiota of Domica Cave in Slovak Karst (Slovakia)
Gaalova B, Donauerova A, Seman M, Bujdakova H 
International Journal of Speleology, 2014, Vol. 43, Iss. 1
Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant Klebsiella pneumoniae Isolate
Al-Marzooq F, Yusof MYM, Tay ST
Jpn. J. Infect. Dis., 2013, Vol 66, No 6, p 555-557
Development and Characterization of Genomic and Gene-Based Microsatellite Markers in North American Red Oak Species
Sullivan AR, Lind JF, McCleary TS, Romero-Severson J, Gailing O
Plant Mol. Biol. Rep. 2013, Vol. 31, pp 231-239
Phylogenetic relationships among host plants explain differences in fungal species richness and community composition in ectomycorrhizal symbiosis
Tedersoo L, Mett M, Ishida TA, Bahram M
New Phytologist, 2013, DOI: 10: 1111/nph. 12328
Comparison of the levels of Equus caballus papillomavirus type 2 (EcPV-2) DNA in equine squamous cell carcinomas and non-cancerous tissues using quantitative PCR
Knighta CG, Dunowska M,  Munday JS, Peters-Kennedy J, Rosa BV
Vetmic. 2013 Vol 166, Iss 1-2, P. 257-262

Sample size: 0.1 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.


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Reg no: 10242922

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50411 Tartu


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