HOT FIREPol® DNA Polymerase

HOT START DNA Polymerase

For in vitro use only



HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.


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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
01-02-00500500 U5 U/μlPDFPDFPDF45 EUR Login to buy!
01-02-010001000 U5 U/μlPDFPDFPDF90 EUR Login to buy!


- Hot Start PCR

- Primer extension

- TA cloning


5 U/µl


Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.


Reagents Provided:

- HOT FIREPol® DNA Polymerase  

- HOT FIREPol® 10x Buffer B1 (Mg2+, detergent free)  Tris-HCl and (NH4)2SO4

- HOT FIREPol® 10x Buffer B2 (Mg2+ free) Tris-HCl, (NH4)2SO4 and detergent

- 25 mM MgCl2

- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® DNA Polymerase.

Please browse through our Troubleshooting Guide for End-Point PCR

send an e-mail directly to our technical support or 

contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

References of HOT FIREPol® DNA Polymerase

Correlation between the human fecal microbiota and depression
Naseribafrouei A, Hestad K, Avershina E, Sekelja M, Linløkken A, Wilson R, Rudi K
Neurogastroenterology & Motility, 2014, Vol. 26, Iss. 8, Pp. 1155–1162

Association of common variants in PAH and LAT1 with non-syndromic cleft lip with or without cleft palate (NSCL/P) in the Polish population
Hozyasz KK, Mostowska AA, Wójcicki P, Lasota A, Wołkowicz A, Dunin-Wilczyńska I, Jagodziński PP
Archives of Oral Biology, Vol. 59, Iss. 4, Pp. 363–369

Light modulation of human sleep depends on a polymorphism in the clock gene Period3
Chellappa Sl, Viola AU, Schmidt C, Bachmann V, Gabel V, Maire M, Reichert CF, Valomon A, Landolt HP, Cajochen C
Behavioural Brain Research, 2014, Vol. 271, Pp. 23–29

Dopaminergic Role in Regulating Neurophysiological Markers of Sleep Homeostasis in Humans
Holst SC, Bersagliere A, Bachmann V, Berger W, Achermann P, Landolt HP
The Journal of Neuroscience, 2014, 34(2): 566-573

Whole-exome sequencing identifies a polymorphism in the BMP5 gene associated with SSRI treatment response in major depression
Tammiste A. et al. 
Journal of Phychopharmacology, 2013, Vol. 27, No 10, Pp. 915-920

Gene-based high-density mapping of the gene rym7 conferring resistance to Barley mild mosaic virus (BaMMV)
Yang P, Perovic D, Habekuss A, Zhou R, Garner A, Ordon F, Stein N
Mol. Breeding, Online. 2012. DOI 10.1007/s11032-013-9842-z

Dopamine transporter genotype predicts attentional asymmetry in healthy adults
Newmana DP, O'Connellb RG, Nathana PJ, Bellgrovea MA
Neuropsychologia, 2012. Vol. 50, Issue 12, Pp. 2823–28

Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America
Barrandeguy ME, Prinz K, García MV, Finkeldey R
Am. J. Bot. September 2012 vol. 99, no. 9, Pp. 372-374

Human Melatonin and Alerting Response to Blue-Enriched Light Depend on a Polymorphism in the Clock Gene PER3
Chellappa SL et al.
The Journal of Clinical Endocrinology & Metabolism. 2012, vol. 97 no. 3, Pp. 433-437

Functional ADA Polymorphism Increases Sleep Depth and Reduces Vigilant Attention in Humans
Bachmann V, Klaus F, Bodenmann S, Schäfer N, Brugger P, Huber S, Berger W, Landolt HP
Cereb. Cortex (2012) 22 (4), Pp. 962-970.

Functional analysis of a lung cancer risk haplotype in the IL1B gene regulatory region
Landvik NE, Hart K, Haugen A, Zienolddin S
Journal of Human Genetics, Sept. 2012

Sample size: 100 U | 5 U/μl

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.


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