Applications:
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
Reagent Composition:
- FIREPol® DNA polymerase
- 5x Reaction Buffer B 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 12.5 mM MgCl2 1x PCR solution – 2.5 mM MgCl2
- 1 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Advantages of Master Mix:
- decreased set-up time
- reduced risk of contamination
- less pipetting mistakes
- consistent reaction to reaction performance
- contains all reagents required for PCR
Like all our products, the 5x FIREPol® Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5x Master Mix.
Please browse through our
Troubleshooting Guide for End-Point PCR,
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.
References of FIREPol® Master Mix
Analysis of Clinical Isolates of Helicobacter pylori in Pakistan Reveals High Degrees of Pathogenicity and High Frequencies of Antibiotic Resistance
Rasheed F, Campbell BJ, AlfizahH, Varro A, Zahra R, Yamaoka Y, Pritchard D
Heliobacter, 2014, Online, DOI:10.1111/hel.12142
Differential Endosomal Pathways for Radically Modified Peptide Vectors
Arukuusk P, Pärnaste L, Margus H, Eriksson NKJ, Vasconcelos L, Padari K, Pooga M, Langel L
Bioconjugate Chem., 2013, 24 (10), pp 1721–1732
CCL5/CCR1 axis regulates multipotency of human adipose tissue derived stromal cells
Kauts ML, Pihelgas S, Orro K, Neuman T, Piirsoo A
Stem Cell Research, 2013, Vol. 10, Iss. 2, Pp 166–178