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5x HOT FIREPol® SolisGreen qPCR Mix

Suitable for ROX-dependent and ROX-independent qPCR cyclers

For in vitro use only


Description:

HOT FIREPol® SolisGreen qPCR Mix is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template and primers.


HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-46-000011 ml5xPDFPDFPDF57 EUR Login to buy!
08-46-000088 ml5xPDFPDFPDF432 EUR Login to buy!
08-46-0002020 ml5xPDFPDFPDF970 EUR Login to buy!

Applications:

- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination 

 

Benefits:

- improved detection of low target concentrations

- higher fluorescence level and lower Ct values

- compatible on most cyclers

 

Mix Compatibility:

- Appilied BioSystems: QuantStudio™ 3, 5, 6, 7, 12K Flex, ViiA™ 7, 7500

- Stratagene: MX3000PTM , MX3005PTM

- Bio-Rad: CFX96™ & CFX384™

- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000 

- Eppendorf: Mastercycler® ep Realplex

- llumina: The Eco™  

- Roche: LightCycler® 480


Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x qPCR buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM 

- dNTPs

- SolisGreen dye 

- Internal reference based on ROX dye 

 

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.


Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® SolisGreen qPCR Mix.

 

FIREPol is a trademark of Solis BioDyne. Applied Biosystems is a trademark of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation. Stratagene, M30005, MX3005P are trademarks of Agilent Technologies inc. Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a trademark of Qiagen GmbH. CFX96 and CFX384 are trademarks of Bio-Rad Laboratories. LightCycler is a trademark of Roche Diagnostics GMBH. The Eco is a trademark of Illumina Inc. 

Please browse through our Troubleshooting Guide for qPCR, 

 
send an e-mail directly to our technical support or 
 
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.
 

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.