5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

For in vitro use only


HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye. The user simply needs to add water, template and primers. 

HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-24-000011 ml5xPDFPDFPDF46.5 EUR Login to buy!
08-24-000088 ml5xPDFPDFPDF352 EUR Login to buy!
08-24-0002020 ml5xPDFPDFPDF810 EUR Login to buy!


- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination 

Mix Compatibility:

- Appilied BiosystemsQuantStudioTM 12K Flex, ViiATM7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM

- Stratagene: MX3000PTM , MX3005PTM

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® qPCR buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM 

- dNTPs

- EvaGreen® dye 

- ROX dye


EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX).


EvaGreen® is a registered trademark of BIOTIUM, INC.
This product is provided under an agreement between BIOTIUM, INC. and SBD and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis.  The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.  Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research.  For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.

Applied BioSystems and ROX are trademarks of Applied Biosystems LLC, ViiA and QuantStudio are trademark of Life Technologies Corporation; Stratagene, MX3000P and MX3005P are trademarks of Stratagene Corporation.

Please browse through our Troubleshooting Guide for qPCR

send an e-mail directly to our technical support or 

contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

References of 5x HOT FIREPol® EvaGreen® qPCR Mix

Expression and localization of locally produced growth factors regulating lymphangiogenesis during different stages of the estrous cycle in corpus luteum of buffalo (Bubalus bubalis)

Ali I. et al.

Theriogenology 2014, Vol. 81, Iss 3, p. 428-436

Downregulation of the glucocorticoid-induced leucine zipper (GILZ) promotes vascular inflammation

Hahn RT, Hoppstädter J, Hirschfelder K, Hachenthal N, Diesel B, Kessler SM, Huwer H, Kiemer AK

Atherosclerosis 2014, Vol 234, Iss 2, P. 391-400

Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice

Ramirez MC,  Zubeldía-Brenner L, Wargon V, Ornstein AM, Becu-Villalobos D

Molecular and Cellular Endocrinology 2014, Vol. 382, Iss 2, p. 825-834

Daidzein Suppresses Tumor Necrosis Factor-α Induced Migration and Invasion by Inhibiting Hedgehog/Gli1 Signaling in Human Breast Cancer Cells

Bao C, Namgung H, Lee J, Park H, Ko J, Moon H, Ko HW, Lee HJ

J. Agric. Food Chem. 2014, 62 (17), pp 3759–3767

The extract of Elaeocarpus sylvestris inhibits human cytomegalovirus immediate early gene expression and replication in vitro

To KP, Kang SC, Song Y

Molecular Medicine Reports 2013, Vol. 9, Iss. 2, p. 744-748

Development of fast multiplex real-time PCR assays based on EvaGreen fluorescence dye for identification of beef and soybean origins in processed sausages

Safdar M, Abasıyanık MF

Food Research International 2013 Dec. Vol 54, Issue 2, Pp 1652-1656

Temporal changes in pregnancy-associated glycoproteins across different stages of gestation in the Barbari goat

Tandiya U. et al.

Animal Reproduction Science 2013, Vol. 142, Iss. 3-4, P. 141-148

Antigen peptide transporter 1 is involved in the development of fructose-induced hepatic steatosis in mice

Arindkar S. et al.

Journal of Gastroenterology and Hepatology 2013, Vol. 28, Iss. 8, p. 1403–1409

Bifidobacterial succession and correlation networks in a large unselected cohort of mothers and their children

Avershina E. et al.

Applied and Environmental Microbiology 2013, Vol. 79, No. 2. P. 497-507

Myrosinases TGG1 and TGG2 from Arabidopsis thaliana contain exclusively oligomannosidic N-glycans

Liebminger E, Grass J, Jez J, Neumann L, Altmann F, Strasser R

Phytochemistry. 2012, Dec. Vol 84, Pp 24-30 

Protective efficacy of Mycobacterium indicus pranii against tubercolosis and underlying local lung immune response in guinea pig model

Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, Katoch VM, Bhaskar S

Vaccine. 2012 21. Sept. Vol 30, Issue 43, Pp 6198–6209

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.


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