5x HOT FIREPol® EvaGreen® qPCR Supermix

Suitable for ROX-dependent and ROX-independent qPCR cyclers

For in vitro use only


HOT FIREPol® EvaGreen® qPCR Supermix is an optimised ready-to-use solution for real-time quantitative PCR assays, including EvaGreen® dye. It comprises all the components necessary to perform qPCR excluding the template and primers, to perform highly sensitive qPCR.

HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-36-000011 ml5xPDFPDFPDF58 EUR Login to buy!
08-36-000088 ml5xPDFPDFPDF452 EUR Login to buy!
08-36-0002020 ml5xPDFPDFPDF1010 EUR Login to buy!


- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination 



- Highly specific and reproducible real time PCR

- Excellent efficiency in case of low copy number targets

- UNG treatment capability due to dNTP blend of dUTP/dTTP

- Superior performance with GC-rich templates

- Blue visualisation dye for easy pipetting


Mix Compatibility:

- Appilied BioSystems: QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM

- Stratagene: MX3000PTM , MX3005PTM

- Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®

- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000 

- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4 

- llumina: The Eco™  

- Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x EvaGreen® qPCR buffer

- 12.5 mM MgCl2 1x PCR solution – 2.5 mM 

- dNTPs blend containing dUTP/dTTP including dTTP to improve reaction sensitivity and efficiency compared to dUTP 

- EvaGreen® dye 

- Internal reference based on ROX dye

- GC-rich Enhancer

- Blue visualisation dye


EvaGreen® Dye:

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!


ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Supermix.


EvaGreen® is a registered trademark of BIOTIUM, INC.
This product is provided under an agreement between BIOTIUM, INC. and SBD and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis.  The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.  Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research.  For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.

FIREPol is a trademark of Solis BioDyne. Applied Biosystems and StepOne are trademarks of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation. Stratagene, M30005, MX3005P are trademarks of Agilent Technologies inc. Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a trademark of Qiagen GmbH. CFX96, CFX384, iQ, MyiQ, Opticon 2, Cromo4, MiniOpticon are trademarks of Bio-Rad Laboratories.  LightCycler is a trademark of Roche Diagnostics GMBH. The Eco is a trademark of Illumina Inc. EvaGreen is a trademark of Biotium Inc. SYBR® is a registered trademark of Molecular Probes, Inc. 

Please browse through our Troubleshooting Guide for qPCR

send an e-mail directly to our technical support or 
contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.

References of 5x HOT FIREPol®  EvaGreen® qPCR Supermix

Adenovirus Precursor pVII Protein Stability Is Regulated By Its Propeptide Sequence
Inturi R, Thaduri S, Punga T
PloS ONE 2013: e80617

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.


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Reg no: 10242922

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