5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

For in vitro use only


HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. 

HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
08-15-000011 ml5xPDFPDFPDF36.5 EUR Login to buy!
08-15-000088 ml5xPDFPDFPDF276 EUR Login to buy!
08-15-0002020 ml5xPDFPDFPDF640 EUR Login to buy!


- Detection and quantification of DNA and cDNA targets

- Profiling gene expression

- Microbial detection

- Viral load determination

Mix Compatibility:

- Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®

- Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

- Eppendorf: Mastercycler®: ep realplex2 & ep realplex4  

- Illumina: The Eco™   

- Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x Probe qPCR buffer

- 15 mM MgCl2 1x PCR solution – 3 mM   

- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP 

- No ROX dye

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (no ROX).


Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.

Please browse through our Troubleshooting Guide for qPCR

send an e-mail directly to our technical support or 

contact technical support via skype: support.sbd. All inquiries will be responded within 24 hours at most.


References of 5x HOT FIREPol® Probe qPCR Mix 

Effect of COMT Val158Met polymorphism on personality traits and educational attainment in a longitudinal population representative study

Lehto K, Akkermann K, Parik J, Veidebaum T, Harro J

European Psychiatry 2013 Oct. Vol. 28. Iss. 8. pp 492-498

Triglyceride metabolism in bone tissue is associated with osteoblast and osteoclast differentiation: a gene expression study

Dragojevic J, Zupan J, Haring G, Herman S, Komadina R, Marc J

J  Bone Miner Metab. 2013. 31:512-519

Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage

Rull K, Tomberg K, Kõks S, Männik J, Möls M, Sirotkina M, Värv S, Laan M.

Placenta. 2013 Feb. Vol 34. Iss 2. pp141-148

The relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human osteoporotic and osteoarthritic

bone tissues.

Zupan J, Komadina R, Marc J.

J. of Biomed. Science. 2012. 19:28

Mid-Gestational Gene Expression Profile in Placenta and Link to Pregnancy Complications

Uusküla L, Männik J, Rull K, Minajeva A, Kõks S, Vaas P, Teesalu P, Reimand J, Laan M

Plos One 2012, DOI: 10.1371/journal.pone.0049248

Proteome changes of human bronchial epithelial cells in response to pro-inflammatory mediator leukotriene E4 and pro-remodelling factor TGF-beta1.

Altraja S, Jaama J, Altraja A.

J Proteomics. 2010 Apr 18;73(6):1230-40. Epub 2010 Feb 26.

Micro RNAexpression profiles of human blood monoyte-derived dendric cells and macrophages reveal miR-511 as a putative positive regulator of TLR4.

Tserel L, Runnel T, Kisand K, Pihlap M, Bakhoff L, Kolde R, PetersonH, Vilo J, Peterson P, Rebane A.

Journal of Biological Chemistry. 2011 June. 286 (30).

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.


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