SolisFAST® SolisGreen® qPCR Mix (no ROX)

Fast and highly sensitive dye-based qPCR

For in vitro use only

 

Description:

SolisFAST™ SolisGreen® qPCR Mix (no ROX) is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays. Combining novel in silico-designed SolisFAST™ DNA Polymerase with fast oligo-based hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up. 

The mix contains all components necessary, except primers, nuclease-free water, and DNA template.

The mix is compatible with qPCR cyclers that do not need ROX as a passive reference signal for normalization of the data.

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Cat. No. Pack size Conc. Data sheet MSDS CoA Price Product qty
28-41-000011 ml5xPDFPDF174 EUR Login to buy!
28-41-00001-55 x 1 ml5xPDFPDF820 EUR Login to buy!
28-41-0002020 ml5xPDFPDF2880 EUR Login to buy!

Applications:

- DNA/cDNA target detection

- Gene expression analysis

- Copy Number Variation (CNV) analysis

- miRNA profiling and quantification

 

Benefits:

  • FAST - delivers reproducible qPCR results up to 2x faster
  • ACCURATE - reliable quantification across technical replicates
  • SENSITIVE - consistent results with low- and high-copy targets
  • TRUSTWORTHY - robust performance and 30 day stability at room temperature for extra convenience

 

Mix Compatibility:

- Bio-Rad: CFX96™, CFX384™, Opticon™, Opticon2™, MiniOpticon, Chromo4™

- Qiagen: Rotor-Gene™ 3000 & 6000

- Eppendorf: Mastercycler® ep realplexMastercycler®

- Roche: LightCycler 480

- Bio Molecular Systems: Mic

- Takara: Thermal Cycler Dice (TP800)

 

Mix Composition:

- SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast oligo-based hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

- 5x qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction

- SolisGreen® dye: fluorescent dye detected in the FAM channel

 

Shipping and Storage Conditions:

  • Routine storage at -20 °C until expiry date
  • The mix can be stored at +4 °C for up to 6 months
  • Stability at room temperature (15 - 25 °C) for 1 month
  • Freeze-thaw stability: 30 cycles

 


This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999.


SolisFAST is a trademark of Solis Biodyne OÜ. This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ. Other registered trademarks: TaqMan® and LightCycler® (Roche Diagnostics GmbH), Mastercycler® (Eppendorf AG). All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

Please contact support@solisbiodyne.com for any questions or comments regarding the performance of the Mix.

Sample size: 0.2 ml | 5x

Order free sample

For research use only.
No components from animal or human origin

Some applications this product is used in may require a license which is not provided by the purchase of this product. Users should obtain the license if required.

Contact:

Solis BioDyne OÜ
Reg no: 10242922

Teaduspargi 9
50411 Tartu
Estonia

E-mail: info@solisbiodyne.com

Tel: +372 740 9960

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